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Volume 1 – Issue 1 – 2020

Original Research Article

Oxidative Changes In Brain Tissue After Concurrent Exposure To Arsenic And Quinalphos In Wistar Rats

Parvinder Singh1, Pawan Kumar Verma1*, Priyanka Sharma1, Shilpa Sood2, Rajinder Raina1

1Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Science and
Animal Husbandry, R S Pura, 181102, (INDIA)
2Division of Veterinary Pathology, Faculty of Veterinary Science and Animal Husbandry, R S Pura, 181102, (INDIA)

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The present study was designed to assess oxidative changes occurring in brain after concurrent exposure of arsenic and quinalphos in Wistar rats. Rats of either sex were randomly allocated to nine groups of six rats each and were administered quinalphos and arsenic either alone or in-conjunction with each other for 28 days. Group I served as control, group II and III received orally quinalphos at 1/100th and 1/10th of LD50 respectively, whereas group IV and V received arsenic 50 and 100 ppb respectively in drinking water. Group VI and VII received low and high dose of quinalphos respectively along with arsenic (50 ppb) in drinking water. Similarly the animals comprising group VIII and IX received higher and lower doses of quinalphos respectively with arsenic (100 ppb) added in drinking water. Significant (P<0.05) declines in brain acetylcholinesterase (AChE), total thiols (TTH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-s-transferase (GST), glutathione reductase (GR) along with significant elevations in (P<0.05) malondialdehyde (MDA) levels pointed towards the occurrence of oxidative damage in brain following repeated administrations of quinalphos at either dose levels or arsenic at the concentration of 100 ppb as compared to control. Moreover, these alterations were found to be more pronounced in groups receiving both treatments concurrently viz. decreased levels of AChE (55.4%), TTH (51.5%), CAT (38.4%), SOD (29.6%), GPx (40.9%), GST (54.9%) and GR (44.4%) with increased MDA (102.3%) as compared to control group. Histopathological changes observed in brain included neuronal degeneration and necrosis, gliosis, neuronophagia and spongiosis which correlated well with dose and co-exposure induced altered antioxidant biomarkers in brain. Hence these findings underline the subtle neurotoxic potential of arsenic and quinalphos which is enhanced with their concurrent exposure.